A biochemical comparison of fungal GH6 cellobiohydrolases.
Cel6A
GH6
cellobiohydrolases
cellulose
enzyme kinetics
hydrolysis
Journal
The Biochemical journal
ISSN: 1470-8728
Titre abrégé: Biochem J
Pays: England
ID NLM: 2984726R
Informations de publication
Date de publication:
05 08 2019
05 08 2019
Historique:
received:
18
03
2019
revised:
14
07
2019
accepted:
16
07
2019
pubmed:
18
7
2019
medline:
19
3
2020
entrez:
18
7
2019
Statut:
epublish
Résumé
Cellobiohydrolases (CBHs) from glycoside hydrolase family 6 (GH6) make up an important part of the secretome in many cellulolytic fungi. They are also of technical interest, particularly because they are part of the enzyme cocktails that are used for the industrial breakdown of lignocellulosic biomass. Nevertheless, functional studies of GH6 CBHs are scarce and focused on a few model enzymes. To elucidate functional breadth among GH6 CBHs, we conducted a comparative biochemical study of seven GH6 CBHs originating from fungi living in different habitats, in addition to one enzyme variant. The enzyme sequences were investigated by phylogenetic analyses to ensure that they were not closely related phylogenetically. The selected enzymes were all heterologously expressed in
Identifiants
pubmed: 31311837
pii: BCJ20190185
doi: 10.1042/BCJ20190185
doi:
Substances chimiques
Fungal Proteins
0
Cellulose 1,4-beta-Cellobiosidase
EC 3.2.1.91
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2157-2172Informations de copyright
© 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.