The Impact of Alkyl-Chain Purity on Lipid-Based Nucleic Acid Delivery Systems - Is the Utilization of Lipid Components with Technical Grade Justified?
gene transfection
lipid dispersions
monolayers
phase transition
structures
Journal
Chemphyschem : a European journal of chemical physics and physical chemistry
ISSN: 1439-7641
Titre abrégé: Chemphyschem
Pays: Germany
ID NLM: 100954211
Informations de publication
Date de publication:
16 08 2019
16 08 2019
Historique:
received:
13
05
2019
revised:
29
06
2019
pubmed:
3
7
2019
medline:
31
8
2019
entrez:
3
7
2019
Statut:
ppublish
Résumé
The physicochemical properties and transfection efficacies of two samples of a cationic lipid have been investigated and compared in 2D (monolayers at the air/liquid interface) and 3D (aqueous bulk dispersions) model systems using different techniques. The samples differ only in their chain composition due to the purity of the oleylamine (chain precursor). Lipid 8 (using the oleylamine of technical grade for cost-efficient synthesis) shows lateral phase separation in the Langmuir layers. However, the amount of attached DNA, determined by IRRAS, is for both samples the same. In 3D systems, lipid 8 p forms cubic phases, which disappear after addition of DNA. At physiological temperatures, both lipids (alone and in mixture with cholesterol) assemble to lamellar aggregates and exhibit comparable DNA delivery efficiency. This study demonstrates that non-lamellar structures are not compulsory for high transfection rates. The results legitimate the utilization of oleyl chains of technical grade in the synthesis of cationic transfection lipids.
Identifiants
pubmed: 31265754
doi: 10.1002/cphc.201900480
pmc: PMC6771585
doi:
Substances chimiques
Amines
0
Lipids
0
Liposomes
0
DNA
9007-49-2
calf thymus DNA
91080-16-9
Cholesterol
97C5T2UQ7J
oleylamine
ZDQ1JWQ8DT
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2110-2121Subventions
Organisme : HASYLAB
Pays : International
Organisme : DESY
Pays : International
Organisme : Max Planck Society
Pays : International
Organisme : European Union
Pays : International
Organisme : Warsaw University of Technology Development Progra
Pays : International
Organisme : Centre for Advanced Studies
Pays : International
Informations de copyright
©2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
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