Characterization of IgE-binding proteins in the salivary glands of Simulium nigrogilvum (Diptera: Simuliidae).
IgE-binding proteins
Simulium dermatitis
Simulium nigrogilvum
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
06
02
2019
accepted:
21
06
2019
pubmed:
3
7
2019
medline:
23
10
2019
entrez:
3
7
2019
Statut:
ppublish
Résumé
Simulium dermatitis is an IgE-mediated skin reaction in animals and humans caused by the bites of black flies. Although Simulium nigrogilvum has been incriminated as the main human-biting black fly species in Thailand, information on its salivary allergens is lacking. Salivary gland extract of S. nigrogilvum females was subjected to sodium dodecylsulfate-polyacrylamide gel electrophoresis, and the separated components were applied onto nitrocellulose membranes for immunoblotting, which was performed by probing the protein blots with sera from 17 individuals who were allergic to the bites of S. nigrogilvum. IgE-reactive protein bands were characterized further by liquid chromatography-mass spectrometry (LC-MS/MS) analysis. Nine protein bands (79, 42, 32, 25, 24, 22, 15, 13, and 11 kDa) were recognized in the serum of the subjects. Four of the nine protein bands (32, 24, 15, and 11 kDa) showed IgE reactivity in all (100%) of the tested sera, and they were identified as salivary secreted antigen 5-related protein, salivary serine protease, erythema protein, and hypothetical secreted protein, respectively. Three other proteins, salivary serine protease (25 kDa), salivary D7 secreted protein (22 kDa), and hypothetical protein (13 kDa), reacted with > 50% of the sera. The relevance of the identified protein bands as allergens needs to be confirmed by using pure recombinant proteins, either in the in vivo skin prick test or in vitro detection of the specific IgE in the serum samples of allergic subjects. This will be useful for the rational design of component-resolved diagnosis and allergen immunotherapy for the allergy mediated by the bites of black flies.
Identifiants
pubmed: 31263951
doi: 10.1007/s00436-019-06383-x
pii: 10.1007/s00436-019-06383-x
doi:
Substances chimiques
Allergens
0
Galectin 3
0
Insect Proteins
0
Immunoglobulin E
37341-29-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2353-2359Subventions
Organisme : Thailand Research Fund (TRF)
ID : MRG5980101
Organisme : Faculty of Medicine Research Fund
ID : PAR-2558-03360
Organisme : Diamond Research Grant
ID : PAR-2560-04663
Organisme : NSTDA Chair Professor Grant
ID : P-1450624
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