Regulation of dendrite morphology and excitatory synapse formation by zDHHC15.
DLG4
Dendrite morphology
Excitatory synapses
PSD-95
Palmitoyl transferases
Palmitoylation
zDHHC15
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
05 07 2019
05 07 2019
Historique:
received:
23
01
2019
accepted:
28
05
2019
pubmed:
14
6
2019
medline:
14
7
2020
entrez:
14
6
2019
Statut:
epublish
Résumé
Protein palmitoylation is the most common post-translational lipid modification in the brain and is mediated by a family of 24 zDHHC enzymes. There has been growing interest in zDHHCs due to mounting evidence that these enzymes play key roles in the development and function of neuronal connections, and the fact that a number of zDHHCs have been associated with neurodevelopmental and neurodegenerative diseases. Loss-of-function variants in several zDHHCs, including zDHHC15, have been identified in patients with intellectual disabilities; however, the function of zDHHC15 in the brain has not been well studied. Here, we demonstrate that knocking down zDHHC15 in primary rat hippocampal cultures reduces dendritic outgrowth and arborization, as well as spine maturation. Moreover, knockdown of zDHHC15 reduces palmitoylation of PSD-95 and its trafficking into dendrites, resulting in an overall decrease in the density of excitatory synapses being formed onto mutant cells.
Identifiants
pubmed: 31189538
pii: jcs.230052
doi: 10.1242/jcs.230052
pmc: PMC6633394
pii:
doi:
Substances chimiques
DNA-Binding Proteins
0
Disks Large Homolog 4 Protein
0
Acyltransferases
EC 2.3.-
Zdhhc15 protein, rat
EC 2.3.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : CIHR
ID : MOP-142721
Pays : Canada
Organisme : CIHR
ID : PJT-148938
Pays : Canada
Informations de copyright
© 2019. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
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