Sac1 Phosphoinositide Phosphatase Regulates Foam Cell Formation by Modulating SR-A Expression in Macrophages.
Sac1
foam cell formation
lipid droplet
macrophage
phosphoinositide phosphatase
scavenger receptor A (SR-A)
Journal
Biological & pharmaceutical bulletin
ISSN: 1347-5215
Titre abrégé: Biol Pharm Bull
Pays: Japan
ID NLM: 9311984
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
4
6
2019
pubmed:
4
6
2019
medline:
26
11
2019
Statut:
ppublish
Résumé
Macrophages endocytose modified low-density lipoproteins (LDL) vigorously via scavenger receptor A (SR-A) to become foam cells. In the present study, we found that Sac1, a member of the Sac family of phosphoinositide phosphatases, increases the protein level of SR-A and upregulates foam cell formation. Mouse macrophages (RAW264.7) were transfected with short hairpin RNAs (shRNAs) against Sac1. Sac1 knockdown decreased cell surface SR-A levels and impaired acetylated LDL-induced foam cell formation. Transfection of Sac1-knockdown cells with shRNA-resistant flag-Sac1 effectively rescued the expression of SR-A. Glycosylation of SR-A was largely attenuated by Sac1 knockdown, but neither mRNA expression nor protein degradation of SR-A were affected. These results suggest that Sac1 maintains SR-A protein levels by modulating SR-A glycosylation.
Identifiants
pubmed: 31155588
doi: 10.1248/bpb.b18-00907
doi:
Substances chimiques
Lipoproteins, LDL
0
Membrane Proteins
0
RNA, Messenger
0
RNA, Small Interfering
0
Sac1 protein, mammalian
0
Scavenger Receptors, Class A
0
Phosphoinositide Phosphatases
EC 3.1.3.36
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM