Retinol binding protein 4 abundance in plasma and tissues is related to body fat deposition in cattle.
Adipocytes
/ chemistry
Adipose Tissue
/ chemistry
Adiposity
Animal Husbandry
Animals
Blood Glucose
/ metabolism
Cattle
/ blood
Fatty Acids
/ analysis
Gene Expression Profiling
Hybridization, Genetic
Immunohistochemistry
Insulin
/ metabolism
Insulin Resistance
Liver
/ chemistry
Male
Muscle, Skeletal
/ chemistry
RNA, Messenger
/ analysis
Red Meat
/ analysis
Retinol-Binding Proteins, Plasma
/ analysis
Reverse Transcriptase Polymerase Chain Reaction
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 05 2019
30 05 2019
Historique:
received:
28
01
2019
accepted:
17
05
2019
entrez:
1
6
2019
pubmed:
31
5
2019
medline:
24
10
2020
Statut:
epublish
Résumé
Retinol binding protein 4 (RBP4) facilitates the transport of retinol in the body but is also an adipokine and fatty acid transporter. Our study was aimed at investigating the associations between RBP4 abundance and fat deposition in cattle. Blood samples of 246 crossbred bulls were taken at 8 months of age and at slaughter at 18 months of age for the determination of RBP4, hormone levels, and fatty acid composition. Significant correlations between plasma RBP4 abundance at 8 months of age and carcass traits at 18 months of age were detected (e.g., r = 0.3; P < 0.001 to carcass fat). Furthermore, RBP4 abundances in the plasma and subcutaneous fat were higher (P < 0.05) in bulls with increased fat deposition, whereas the liver RBP4 expression was not (P > 0.05). Retinol binding protein 4 was immunohistochemically localized in or close to adipocytes within muscle and adipose tissue and in liver stellate cells but not in hepatocytes. Overall, our results indicate that increased RBP4 levels were associated with increased fat deposition and altered fatty acid composition, but not with altered glucose tolerance, in crossbred bulls. Moreover, our results suggest that adipose-tissue-derived RBP4 may contribute to the circulating RBP4 level.
Identifiants
pubmed: 31147589
doi: 10.1038/s41598-019-44509-4
pii: 10.1038/s41598-019-44509-4
pmc: PMC6542835
doi:
Substances chimiques
Blood Glucose
0
Fatty Acids
0
Insulin
0
RNA, Messenger
0
Retinol-Binding Proteins, Plasma
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8056Références
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