Regenerative Features of Adipose Tissue for Osteoarthritis Treatment in a Rabbit Model: Enzymatic Digestion Versus Mechanical Disruption.
CD-163 macrophages
adipose niche
cartilage
expanded adipose-derived stromal cells
local biodistribution
meniscus
osteoarthritis
synovial membrane
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
29 May 2019
29 May 2019
Historique:
received:
12
04
2019
revised:
19
05
2019
accepted:
23
05
2019
entrez:
1
6
2019
pubmed:
31
5
2019
medline:
15
11
2019
Statut:
epublish
Résumé
Evaluating cell migration after cell-based treatment is important for several disorders, including osteoarthritis (OA), as it might influence the clinical outcome. This research explores migrating expanded-adipose stromal cells (ASCs) and adipose niches after enzymatic and mechanical processes. Bilateral anterior cruciate ligament transection induced a mild grade of OA at eight weeks in adult male New Zealand rabbits. ASCs, enzymatic stromal vascular fraction (SVF), and micro fragmented adipose tissue (MFAT) were intra-articularly injected in the knee joint. Assessments of cell viability and expression of specific markers, including CD-163 wound-healing macrophages, were done. Cell migration was explored through labelling with PKH26 dye at 7 and 30 days alongside co-localization analyses for CD-146. All cells showed good viability and high percentages of CD-90 and CD-146. CD-163 was significantly higher in MFAT compared to SVF. Distinct migratory potential and time-dependent effects were observed among cell-based treatments. At day 7, both ASCs and SVF migrated towards synovium, whereas for MFAT versus cartilage, a different migration pattern was noticed at day 30. The long-term distinct cell migration of ASCs, SVF, and MFAT open interesting clinical insights on their potential use for OA treatment. Moreover, the highest expression of CD-163 in MFAT, rather than SVF, might have an important role in directly mediating cartilage tissue repair responses.
Identifiants
pubmed: 31146351
pii: ijms20112636
doi: 10.3390/ijms20112636
pmc: PMC6601012
pii:
doi:
Substances chimiques
Antigens, Differentiation
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministero della Salute
ID : RF-2011-02352638
Références
Circulation. 2004 Mar 16;109(10):1292-8
pubmed: 14993122
Br J Haematol. 2005 Apr;129(1):118-29
pubmed: 15801964
Stem Cells. 2006 Nov;24(11):2582-91
pubmed: 16873762
Stem Cells. 2007 Apr;25(4):818-27
pubmed: 17420225
Curr Opin Rheumatol. 2007 Sep;19(5):457-62
pubmed: 17762611
Int J Dev Biol. 2008;52(8):1033-42
pubmed: 18956336
J Orthop Res. 2009 Sep;27(9):1197-203
pubmed: 19242978
Nat Rev Immunol. 2009 Apr;9(4):259-70
pubmed: 19282852
Stem Cell Res Ther. 2010 Jun 29;1(2):19
pubmed: 20587076
Osteoarthritis Cartilage. 2010 Oct;18 Suppl 3:S53-65
pubmed: 20864023
Annu Rev Pathol. 2011;6:275-97
pubmed: 21034223
Osteoarthritis Cartilage. 2011 Sep;19(9):1132-41
pubmed: 21683797
Nat Rev Immunol. 2011 Oct 10;11(11):738-49
pubmed: 21984069
J Transl Med. 2011 Oct 21;9:181
pubmed: 22017805
Blood. 2011 Dec 15;118(25):e184-91
pubmed: 22034631
J Clin Invest. 2012 Mar;122(3):787-95
pubmed: 22378047
Bone. 2012 Aug;51(2):249-57
pubmed: 22387238
Expert Opin Biol Ther. 2012 Dec;12(12):1575-88
pubmed: 22953993
Cell Transplant. 2013;22(11):2063-77
pubmed: 23051701
Arthritis Res Ther. 2013 Jan 29;15(1):R22
pubmed: 23360790
Cytotherapy. 2013 Jun;15(6):641-8
pubmed: 23570660
Arthritis Rheum. 2013 May;65(5):1271-81
pubmed: 23613363
Cytotherapy. 2013 Aug;15(8):979-85
pubmed: 23725689
Stem Cell Res. 2013 Sep;11(2):834-44
pubmed: 23811540
Cell Metab. 2013 Sep 3;18(3):355-67
pubmed: 24011071
Stem Cells Int. 2013;2013:678063
pubmed: 24222773
Stem Cells Dev. 2014 Oct 1;23(19):2390-400
pubmed: 24805167
Osteoarthritis Cartilage. 2014 Nov;22(11):1826-32
pubmed: 25084132
Int J Exp Pathol. 2015 Feb;96(1):63-72
pubmed: 25649907
Stem Cells Int. 2015;2015:597652
pubmed: 26240572
Cell Regen (Lond). 2015 Sep 30;4:7
pubmed: 26435835
Springerplus. 2015 Nov 23;4:713
pubmed: 26636001
Curr Stem Cell Rep. 2016;2:304-312
pubmed: 27547712
J Cell Physiol. 2017 Jun;232(6):1478-1488
pubmed: 27739057
J Orthop Res. 2017 Apr;35(4):735-739
pubmed: 27808445
J Biomed Sci. 2017 Jan 31;24(1):9
pubmed: 28143470
Osteoarthritis Cartilage. 2017 Jul;25(7):1161-1171
pubmed: 28153787
SICOT J. 2017;3:43
pubmed: 28589877
Stem Cell Res Ther. 2017 Jun 15;8(1):145
pubmed: 28619097
Arthritis Res Ther. 2017 Jul 5;19(1):157
pubmed: 28679445
Stem Cells. 2017 Oct;35(10):2198-2207
pubmed: 28801931
J Exp Orthop. 2017 Oct 3;4(1):33
pubmed: 28975547
Genes (Basel). 2017 Oct 13;8(10):null
pubmed: 29027984
Tissue Eng Part C Methods. 2018 Feb;24(2):121-133
pubmed: 29108480
J Gene Med. 2018 Jan;20(1):
pubmed: 29243283
Front Med (Lausanne). 2018 Apr 30;5:118
pubmed: 29761103
Stem Cells. 2018 Sep;36(9):1311-1328
pubmed: 29761573
BMC Musculoskelet Disord. 2018 May 30;19(1):176
pubmed: 29848328
BMJ Case Rep. 2018 Jun 4;2018:null
pubmed: 29866683
Stem Cells Transl Med. 2018 Nov;7(11):819-828
pubmed: 30035380
Int J Mol Sci. 2018 Jul 23;19(7):null
pubmed: 30041472
Int J Mol Sci. 2018 Aug 09;19(8):null
pubmed: 30096908
J Med Case Rep. 2018 Aug 29;12(1):238
pubmed: 30153860
J Cell Physiol. 2019 Apr;234(4):5044-5055
pubmed: 30187478
Stem Cells Transl Med. 2018 Dec;7(12):876-886
pubmed: 30255987
Stem Cells Int. 2019 Jan 3;2019:1726743
pubmed: 30719047
Stem Cells Int. 2019 Feb 19;2019:5901479
pubmed: 30915125
Stem Cells. 2019 Jul;37(7):855-864
pubmed: 30977255
Osteoarthritis Cartilage. 1996 Jun;4(2):87-98
pubmed: 8806111