Tooth agenesis-related GLI2 and GLI3 genes may contribute to craniofacial skeletal morphology in humans.
Craniofacial morphology
Genetic variants
Single nucleotide polymorphisms
Skeletal class
Tooth agenesis
Journal
Archives of oral biology
ISSN: 1879-1506
Titre abrégé: Arch Oral Biol
Pays: England
ID NLM: 0116711
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
17
02
2019
revised:
03
05
2019
accepted:
09
05
2019
pubmed:
22
5
2019
medline:
30
1
2020
entrez:
22
5
2019
Statut:
ppublish
Résumé
The present cross-sectional, multi-centre, genetic study aimed to determine, whether single nucleotide polymorphisms (SNPs) in tooth agenesis (TA)-associated GLI2 and GLI3 genes contribute to the development of craniofacial skeletal morphology in humans. Orthodontic patients from an ethnically heterogeneous population were selected for the present study (n = 594). The presence or absence of TA was determined by analysis of panoramic radiography and dental records. The subjects were classified according to their skeletal malocclusion and facial growth pattern by means of digital cephalometric analysis. Genomic DNA was extracted from squamous epithelial cells of the buccal mucosa and SNPs in GLI2 (rs3738880, rs2278741) and GLI3 (rs929387, rs846266) were analysed by polymerase chain reaction using TaqMan chemistry and end-point analysis. Class II skeletal malocclusion presented a significantly lower frequency of TA (P < 0.05). Subjects without TA showed significantly higher ANB angles (P < 0.05). Genotype and/or allele distributions of the SNPs in GLI2 (rs3738880, rs2278741) and GLI3 (rs846266) were associated with the presence of TA (P < 0.05). The SNPs rs3738880, rs2278741 and rs929387 were also associated with some type of skeletal malocclusion (P < 0.05), but not with the facial growth pattern (P > 0.05). The G allele for TA-related GLI2 rs3738880 was strongly linked to the presence of Class III skeletal malocclusion (OR = 2.03; 95% CI = 1.37-3.03; P<3125 × 10 The present study suggests that SNPs in TA-associated GLI2 and GLI3 genes may also play a role in the development of skeletal malocclusions. rs3738880 and rs2278741 in GLI2 seems to contribute to the genetic background for skeletal Class III and TA, respectively. TA could be an additional predictor of craniofacial morphology in some cases. Further research replicating the reported associations should be performed.
Identifiants
pubmed: 31112935
pii: S0003-9969(19)30157-8
doi: 10.1016/j.archoralbio.2019.05.008
pii:
doi:
Substances chimiques
GLI2 protein, human
0
GLI3 protein, human
0
Nerve Tissue Proteins
0
Nuclear Proteins
0
Zinc Finger Protein Gli2
0
Zinc Finger Protein Gli3
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
12-18Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.