A DNA Aptamer for Cyclic Adenosine Monophosphate that Shows Adaptive Recognition.

Aptamers, Nucleotide / chemistry Cyclic AMP / analysis Nucleic Acid Conformation SELEX Aptamer Technique
G-quadruplexes adaptive recognition aptamers cyclic adenosine monophosphate nucleic acids

Journal

Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360

Informations de publication

Date de publication:
15 01 2020
Historique:
received: 19 04 2019
pubmed: 18 5 2019
medline: 6 1 2021
entrez: 18 5 2019
Statut: ppublish

Résumé

As a ubiquitous second messenger, cyclic adenosine monophosphate (cAMP) mediates diverse biological processes such as cell growth, inflammation, and metabolism. The ability to probe these pathways would be significantly enhanced if we had a DNA-based sensor for cAMP. Herein, we describe a new, 31-base long single-stranded DNA aptamer for cAMP, denoted caDNApt-1, that was isolated by in vitro selection using systemic evolution of ligands after exponential enrichment (SELEX). caDNApt-1 has an approximately threefold higher affinity for cAMP than ATP, ADP, and AMP. Using non-denaturing gel electrophoresis and fluorescence spectroscopy, we characterized the structural changes caDNApt-1 undergoes upon binding to cAMP and revealed its potential as a cAMP sensor.

Identifiants

DOI: 10.1002/cbic.201900259 PMID: 31099939 PMC: PMC6960355
pubmed: 31099939
doi: 10.1002/cbic.201900259
pmc: PMC6960355
mid: NIHMS1064645
doi:

Substances chimiques

Aptamers, Nucleotide 0
Cyclic AMP E0399OZS9N

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

157-162

Subventions

Organisme : NINDS NIH HHS
ID : R21 NS114428
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008720
Pays : United States

Informations de copyright

© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Auteurs

Suruchi Sharma (S)

Tata Institute of Fundamental Research, GKVK, National Centre for Biological Sciences, Bellary Road, Bengaluru, 560065, India.

Matthew Zajac (M)

Present address: Department of Chemistry, The University of Chicago, GCIS E305A, 929E, 57th Street, ., Chicago, IL, 60637, USA.

Yamuna Krishnan (Y)

Tata Institute of Fundamental Research, GKVK, National Centre for Biological Sciences, Bellary Road, Bengaluru, 560065, India.
Present address: Department of Chemistry, The University of Chicago, GCIS E305A, 929E, 57th Street, ., Chicago, IL, 60637, USA.
Grossman Institute of Neuroscience, Quantitative Biology and, Human Behavior, University of Chicago, Chicago, IL, 60637, USA.
ORCID: 0000-0001-5282-8852

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Classifications MeSH

Recherchemedicale.com › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › A DNA Aptamer for Cyclic Adenosine...
Recherchemedicale.com › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › AMP cyclique › A DNA Aptamer for Cyclic Adenosine...
Recherchemedicale.com › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › A DNA Aptamer for Cyclic Adenosine...
Recherchemedicale.com › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Techniques de chimie combinatoire › Technique SELEX › A DNA Aptamer for Cyclic Adenosine...