CRISPR Cpf1 proteins: structure, function and implications for genome editing.
Base editing
CRISPR/Cas9
CRISPR/Cpf1
Epigenetic
Gene editing
Transcription
Journal
Cell & bioscience
ISSN: 2045-3701
Titre abrégé: Cell Biosci
Pays: England
ID NLM: 101561195
Informations de publication
Date de publication:
2019
2019
Historique:
received:
13
02
2019
accepted:
20
04
2019
entrez:
16
5
2019
pubmed:
16
5
2019
medline:
16
5
2019
Statut:
epublish
Résumé
CRISPR and CRISPR-associated (Cas) protein, as components of microbial adaptive immune system, allows biologists to edit genomic DNA in a precise and specific way. CRISPR-Cas systems are classified into two main classes and six types. Cpf1 is a putative type V (class II) CRISPR effector, which can be programmed with a CRISPR RNA to bind and cleave complementary DNA targets. Cpf1 has recently emerged as an alternative for Cas9, due to its distinct features such as the ability to target T-rich motifs, no need for trans-activating crRNA, inducing a staggered double-strand break and potential for both RNA processing and DNA nuclease activity. In this review, we attempt to discuss the evolutionary origins, basic architectures, and molecular mechanisms of Cpf1 family proteins, as well as crRNA designing and delivery strategies. We will also describe the novel Cpf1 variants, which have broadened the versatility and feasibility of this system in genome editing, transcription regulation, epigenetic modulation, and base editing. Finally, we will be reviewing the recent studies on utilization of Cpf1as a molecular tool for genome editing.
Identifiants
pubmed: 31086658
doi: 10.1186/s13578-019-0298-7
pii: 298
pmc: PMC6507119
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
36Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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