Efficient Site-Specific Antibody-Drug Conjugation by Engineering a Nature-Derived Recognition Tag for Microbial Transglutaminase.
Animals
Antineoplastic Agents, Immunological
/ chemistry
Bacterial Proteins
/ chemistry
CHO Cells
Cell Line, Tumor
Cricetulus
Humans
Immunoconjugates
/ chemistry
Oligopeptides
/ chemistry
Protein Engineering
Streptomyces
/ enzymology
Substrate Specificity
Transglutaminases
/ chemistry
Trastuzumab
/ chemistry
antibody-drug conjugates
bioconjugation
microbial transglutaminase
protein labeling
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
16 09 2019
16 09 2019
Historique:
received:
18
02
2019
revised:
28
04
2019
pubmed:
3
5
2019
medline:
22
9
2020
entrez:
3
5
2019
Statut:
ppublish
Résumé
Microbial transglutaminase (mTG) has recently emerged as a powerful tool for antibody engineering. In nature, it catalyzes the formation of amide bonds between glutamine side chains and primary amines. Being applied to numerous research fields from material sciences to medicine, mTG enables efficient site-specific conjugation of molecular architectures that possess suitable recognition motifs. In monoclonal antibodies, the lack of native transamidation sites is bypassed by incorporating specific peptide recognition sequences. Herein, we report a rapid and efficient mTG-catalyzed bioconjugation that relies on a novel recognition motif derived from its native substrate Streptomyces papain inhibitor (SPI
Identifiants
pubmed: 31044494
doi: 10.1002/cbic.201900101
doi:
Substances chimiques
Antineoplastic Agents, Immunological
0
Bacterial Proteins
0
Immunoconjugates
0
Oligopeptides
0
Transglutaminases
EC 2.3.2.13
Trastuzumab
P188ANX8CK
monomethyl auristatin E
V7I58RC5EJ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2411-2419Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : NANOKAT II
Pays : International
Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
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