Genetic diversity and multiple antibiotic resistance index study of bacterial pathogen, Klebsiella pneumoniae strains isolated from diseased Indian major carps.
Animals
Anti-Bacterial Agents
/ pharmacology
Aquaculture
Carps
/ microbiology
DNA, Bacterial
/ genetics
DNA, Ribosomal Spacer
/ genetics
Drug Resistance, Multiple, Bacterial
Fish Diseases
/ microbiology
Genes, Bacterial
/ genetics
Genetic Variation
Hemolysis
India
/ epidemiology
Klebsiella Infections
/ epidemiology
Klebsiella pneumoniae
/ classification
Microbial Sensitivity Tests
Phylogeny
Prevalence
RNA, Ribosomal, 16S
/ genetics
Ribotyping
Sequence Analysis, DNA
Antibiotic resistance index
Carp fish
Genetic diversity
India
Klebsiella pneumoniae
Journal
Folia microbiologica
ISSN: 1874-9356
Titre abrégé: Folia Microbiol (Praha)
Pays: United States
ID NLM: 0376757
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
01
10
2018
accepted:
25
03
2019
pubmed:
26
4
2019
medline:
7
3
2020
entrez:
26
4
2019
Statut:
ppublish
Résumé
Intensive fish farming systems have led to increase in disease incidence, due to higher stocking density, high organic matter levels, and poor quality of the aquatic environment. Diseased fish samples showing hemorrhages and reddish lesions were collected from different freshwater fish farms located at three different districts of West Bengal, India (Burdwan, North 24 Parganas, and Nadia). The present study was conducted to evaluate the genetic diversity of ten different Klebsiella pneumoniae strains isolated from different infected freshwater fish samples based on 16S rRNA gene sequence analysis. Primarily, Klebsiella-specific media was used for the isolation and characterization of Klebsiella pneumoniae. Further, through a biochemical test, all the strains were confirmed as K. pneumoniae. PCR analysis of 16S-23S internal transcribed spacer (PCR ribotyping) was carried out to study the species variation within different Klebsiella pneumoniae isolates. For all the isolates, a conserved PCR ribotype pattern was observed while differing from other bacterial species. Phylogenetic study showed the high degree of homology with diverse source of other strains. The multiple antibiotic resistance (MAR) values of the present study for the isolates were found to be 0.468. MAR value above 0.2 indicates that the source of isolation was highly contaminated with antibiotics. Based on the 16S rRNA gene sequence analysis, the present study revealed the genetic diversity of Klebsiella pneumoniae isolated from the different diseased fish farms of West Bengal. All the strains were found to be hypermucoviscous and multidrug-resistant, thus making it pathogenic towards the host organisms. Further, the study revealed a high prevalence of K. pneumoniae in aquaculture farms, representing a risk towards successful aquaculture.
Identifiants
pubmed: 31020632
doi: 10.1007/s12223-019-00701-7
pii: 10.1007/s12223-019-00701-7
doi:
Substances chimiques
Anti-Bacterial Agents
0
DNA, Bacterial
0
DNA, Ribosomal Spacer
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
875-887Subventions
Organisme : NFDB
ID : G/Nat. Surveillance/2013 dated 16.08.2013
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