[Determination of thresholds values for platelet serotonin and urinary 5-HIAA concentrations for the biological diagnosis of digestive neuroendocrine tumors].

Quelles valeurs de référence pour la concentration de la sérotonine plaquettaire et du 5-HIAA urinaire pour le diagnostic des tumeurs neuroendocrines digestives ?

Journal

Annales de biologie clinique
ISSN: 1950-6112
Titre abrégé: Ann Biol Clin (Paris)
Pays: France
ID NLM: 2984690R

Informations de publication

Date de publication:
01 04 2019
Historique:
entrez: 19 4 2019
pubmed: 19 4 2019
medline: 23 8 2019
Statut: ppublish

Résumé

Platelet serotonin and its urinary metabolite 5-HIAA (5-hydroxyindolacetic acid) are the main biomarkers measured for the detection of neuroendocrine tumors (NET). We observe in our laboratory many false positives or false negatives for the 2 assays using threshold values given by the manufacturer. We aim to determine our own local threshold values for a better detection of gastrointestinal NETs. We studied patients with measurement of platelet serotonin and/or urinary 5-HIAA in University Hospital of Tours between January 2005 and June 2016. We established an « index » cohort with 75% of patients to determine local threshold value for the 2 parameters. A "validation" cohort constituted with 25% of remaining patients allowed us to compare the performances of manufacturer's values with local threshold values. Two hundred ninety patients were included, with 19 suffering from NETs. Local threshold value for platelet serotonin was determined at 5.13 amol/platelet, the one for urinary 5-HIAA at 3.60 μmol/mmol urinary creatinine. Platelet serotonin specificity was better with local threshold value for identical sensibility (0.75). Urinary 5-HIAA sensibility was improved with local threshold value (1 vs 0.667) for identical specificity (0.902). Using our local threshold value for platelet serotonin and urinary 5-HIAA improved diagnostic performances of these biochemical markers to detect NETs.

Identifiants

pubmed: 30998196
pii: abc.2019.1432
doi: 10.1684/abc.2019.1432
doi:

Substances chimiques

Biomarkers 0
Serotonin 333DO1RDJY
Hydroxyindoleacetic Acid 54-16-0

Types de publication

Journal Article Validation Study

Langues

fre

Pagination

161-168

Auteurs

Maël Padelli (M)

Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France.

Clément Bruno (C)

Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.

Julia Lemarchand (J)

Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France.

Patrick Vourc'h (P)

Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.

Christian Andres (C)

Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.

Hélène Blasco (H)

Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.

Isabelle Benz-de Bretagne (I)

Laboratoire de biochimie et biologie moléculaire, CHRU de Tours, France, UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.

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Classifications MeSH