The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks.
Animals
Ducks
/ virology
Enzyme-Linked Immunosorbent Assay
/ veterinary
Female
Flavivirus
/ physiology
Flavivirus Infections
/ veterinary
Fluorescent Antibody Technique
/ veterinary
Microscopy, Electron, Transmission
/ veterinary
Poultry Diseases
/ virology
Real-Time Polymerase Chain Reaction
/ veterinary
Spleen
/ virology
Viral Load
/ veterinary
Duck Tembusu virus
Dynamic distribution
Spleen
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
11 Apr 2019
11 Apr 2019
Historique:
received:
05
09
2018
accepted:
03
04
2019
entrez:
13
4
2019
pubmed:
13
4
2019
medline:
30
4
2019
Statut:
epublish
Résumé
Duck Tembusu virus (DTMUV) is a novel member of Flavivirus. The isolated and purified DTMUV strain XZ-2012 was used as a strain model, to intramuscularly inject the six-month egg-laying shelducks with the infective dose of 10 The results showed that the virus occurred in the spleen after 2 hpi and lasted up to 18 dpi. The registered viral load increased from 2 hpi to 3 dpi, and then it diminished from 6 dpi to 18 dpi with a slight rise at 12 dpi. From 2 hpi to 6 dpi the DTMUV particles were mostly distributed in the periellipsoidal lymphatic sheath (PELS) of spleen white pulp, few being found in the sheathed capillary. From 9 dpi to 18 dpi, the DTMUV particles were migrating into periarterial lymphatic sheaths (PALS) around the central artery through the red pulp. Under TEM, the virus particles could be observed mostly in lymphocytes and macrophages. It was suggested that DTMUV invaded lymphocytes and macrophages of the spleen at 2 hpi and replicated significantly from 1 dpi to 3 dpi, being eliminated from 9 dpi to 18 dpi. This is the first study on the dynamic distribution of DTMUV from invasion to elimination in duck spleen conducted by molecular and morphological methods. It could provide theoretical basis for the occurrence, development and detoxification of the virus in the organs of the immune system.
Sections du résumé
BACKGROUND
BACKGROUND
Duck Tembusu virus (DTMUV) is a novel member of Flavivirus. The isolated and purified DTMUV strain XZ-2012 was used as a strain model, to intramuscularly inject the six-month egg-laying shelducks with the infective dose of 10
RESULT
RESULTS
The results showed that the virus occurred in the spleen after 2 hpi and lasted up to 18 dpi. The registered viral load increased from 2 hpi to 3 dpi, and then it diminished from 6 dpi to 18 dpi with a slight rise at 12 dpi. From 2 hpi to 6 dpi the DTMUV particles were mostly distributed in the periellipsoidal lymphatic sheath (PELS) of spleen white pulp, few being found in the sheathed capillary. From 9 dpi to 18 dpi, the DTMUV particles were migrating into periarterial lymphatic sheaths (PALS) around the central artery through the red pulp. Under TEM, the virus particles could be observed mostly in lymphocytes and macrophages.
CONCLUSION
CONCLUSIONS
It was suggested that DTMUV invaded lymphocytes and macrophages of the spleen at 2 hpi and replicated significantly from 1 dpi to 3 dpi, being eliminated from 9 dpi to 18 dpi. This is the first study on the dynamic distribution of DTMUV from invasion to elimination in duck spleen conducted by molecular and morphological methods. It could provide theoretical basis for the occurrence, development and detoxification of the virus in the organs of the immune system.
Identifiants
pubmed: 30975151
doi: 10.1186/s12917-019-1860-6
pii: 10.1186/s12917-019-1860-6
pmc: PMC6460551
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112Subventions
Organisme : National Natural Science Foundation of China
ID : 31672505
Organisme : National Natural Science Foundation of China
ID : 31872433
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