Reference set of Mycobacterium tuberculosis clinical strains: A tool for research and product development.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2019
Historique:
received: 22 10 2018
accepted: 06 03 2019
entrez: 26 3 2019
pubmed: 26 3 2019
medline: 18 12 2019
Statut: epublish

Résumé

The Mycobacterium tuberculosis complex (MTBC) causes tuberculosis (TB) in humans and various other mammals. The human-adapted members of the MTBC comprise seven phylogenetic lineages that differ in their geographical distribution. There is growing evidence that this phylogeographic diversity modulates the outcome of TB infection and disease. For decades, TB research and development has focused on the two canonical MTBC laboratory strains H37Rv and Erdman, both of which belong to Lineage 4. Relying on only a few laboratory-adapted strains can be misleading as study results might not be directly transferrable to clinical settings where patients are infected with a diverse array of strains, including drug-resistant variants. Here, we argue for the need to expand TB research and development by incorporating the phylogenetic diversity of the MTBC. To facilitate such work, we have assembled a group of 20 genetically well-characterized clinical strains representing the seven known human-adapted MTBC lineages. With the "MTBC clinical strains reference set" we aim to provide a standardized resource for the TB community. We hope it will enable more direct comparisons between studies that explore the physiology of MTBC beyond the laboratory strains used thus far. We anticipate that detailed phenotypic analyses of this reference strain set will increase our understanding of TB biology and assist in the development of new control tools that are broadly effective.

Identifiants

pubmed: 30908506
doi: 10.1371/journal.pone.0214088
pii: PONE-D-18-30522
pmc: PMC6433267
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0214088

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Sònia Borrell (S)

Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.

Andrej Trauner (A)

Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.

Daniela Brites (D)

Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.

Leen Rigouts (L)

Mycobacteriology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Collection of Mycobacterial Cultures (BCCM/ITM), Institute of Tropical Medicine, Antwerp, Belgium.

Chloe Loiseau (C)

Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.

Mireia Coscolla (M)

Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.

Stefan Niemann (S)

Division of Molecular and Experimental Mycobacteriology Group, Research Center Borstel, Borstel, Germany.

Bouke De Jong (B)

Mycobacteriology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.

Dorothy Yeboah-Manu (D)

Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.

Midori Kato-Maeda (M)

School of Medicine, University of California at San Francisco, San Francisco, California, United States of America.

Julia Feldmann (J)

Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.

Miriam Reinhard (M)

Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.

Christian Beisel (C)

Genomics Facility, Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.

Sebastien Gagneux (S)

Swiss Tropical and Public Health Institute, Basel, Switzerland.
University of Basel, Basel, Switzerland.

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Classifications MeSH