Monitoring of the field application of Metarhizium anisopliae in Brazil revealed high molecular diversity of Metarhizium spp in insects, soil and sugarcane roots.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 03 2019
14 03 2019
Historique:
received:
20
07
2018
accepted:
20
12
2018
entrez:
16
3
2019
pubmed:
16
3
2019
medline:
3
11
2020
Statut:
epublish
Résumé
The use of Metarhizium against sugarcane spittlebugs in Brazil is one of the most successful and long lasting biological control programs using entomopathogenic fungus in the world. However, studies to monitor the fate of this fungus on the sugarcane agroecosystem are rare, especially with respect to its persistence, efficacy in pest control and impact on the local populations of Metarhizium. The present study aimed at documenting the efficacy and persistence of M. anisopliae strain ESALQ1604 in a sugarcane field by using microsatellite molecular markers. The species diversity of Metarhizium was characterized in insects, soil and sugarcane roots in a sprayed and an unsprayed plot. Although the infection rates were not very high (≤ 50%), the applied strain was recovered from spittlebugs after 7, 30 and 60 days' post-application, but accounted for only 50%, 50% and 70.5% of all insects killed by M. anisopliae, respectively. All haplotypes from spittlebug were associated with a single subclade of M. anisopliae. The highest haplotype diversity was found in soil (h = 0.989) and in the smallest in spittlebug (h = 0.779). Metarhizium robertsii, M. anisopliae, M. brunneum; one taxonomically unassigned lineage was found in soil and only M. brunneum and M. anisopliae were isolated from roots. This study revealed the great diversity of Metarhizium spp. in the sugarcane agroecosystem and the importance of the local population of M. anisopliae on spittlebugs management.
Identifiants
pubmed: 30872601
doi: 10.1038/s41598-019-38594-8
pii: 10.1038/s41598-019-38594-8
pmc: PMC6418095
doi:
Substances chimiques
Biological Control Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4443Références
Braz J Microbiol. 2016 Dec;47 Suppl 1:64-76
pubmed: 27818090
Mycol Res. 2006 Feb;110(Pt 2):188-95
pubmed: 16378721
Neotrop Entomol. 2013 Aug;42(4):436-8
pubmed: 23949866
J Invertebr Pathol. 2017 Sep;148:60-66
pubmed: 28578154
World J Microbiol Biotechnol. 2012 Apr;28(4):1483-94
pubmed: 22805930
Microbiology (Reading). 2011 Oct;157(Pt 10):2904-2911
pubmed: 21778205
J Basic Microbiol. 2013 Mar;53(3):251-9
pubmed: 22733433
Mycologia. 2014 Jul-Aug;106(4):811-29
pubmed: 24891418
Mycologia. 2009 Jul-Aug;101(4):512-30
pubmed: 19623931
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Plant Mol Biol. 2016 Apr;90(6):657-64
pubmed: 26644135
Fungal Biol. 2016 May;120(5):655-65
pubmed: 27109363
J Invertebr Pathol. 2014 Nov;123:6-12
pubmed: 25224815
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4384-8
pubmed: 8506277
Genetics. 1980 Oct;96(2):523-36
pubmed: 17249067
Adv Appl Microbiol. 2004;54:1-70
pubmed: 15251275
J Invertebr Pathol. 2015 Nov;132:142-148
pubmed: 26407950
Bioinformatics. 2012 Oct 1;28(19):2537-9
pubmed: 22820204
Science. 2012 Jun 22;336(6088):1576-7
pubmed: 22723421
Syst Biol. 2008 Oct;57(5):758-71
pubmed: 18853362
Nat Biotechnol. 2000 Feb;18(2):233-4
pubmed: 10657137
Ecology. 1971 Jul;52(4):577-586
pubmed: 28973811
Neotrop Entomol. 2016 Feb;45(1):80-7
pubmed: 26438409
Environ Microbiol. 2015 Aug;17(8):2791-804
pubmed: 25627647
J Invertebr Pathol. 2019 Jul;165:46-53
pubmed: 29339191
Mycologia. 2014 Jul-Aug;106(4):865-78
pubmed: 24987123
PeerJ. 2014 Mar 04;2:e281
pubmed: 24688859
J Invertebr Pathol. 2018 Jan;151:165-168
pubmed: 29224975
Microbiology (Reading). 2011 Jan;157(Pt 1):47-55
pubmed: 20947574
Appl Environ Microbiol. 2014 Mar;80(5):1553-60
pubmed: 24334669
Neotrop Entomol. 2014 Feb;43(1):90-5
pubmed: 27193410
Mycologia. 2006 Sep-Oct;98(5):737-45
pubmed: 17256577
Microb Ecol. 2017 Jul;74(1):194-206
pubmed: 28124726
Mycol Res. 2009 Sep;113(Pt 9):1016-24
pubmed: 19540917
PLoS Genet. 2011 Jan 06;7(1):e1001264
pubmed: 21253567
Mol Ecol. 2007 Apr;16(8):1701-11
pubmed: 17402984
Am J Bot. 2012 Jan;99(1):101-7
pubmed: 22174335