Seasonality of Planktonic Freshwater Ciliates: Are Analyses Based on V9 Regions of the 18S rRNA Gene Correlated With Morphospecies Counts?
Lake Zurich
V9 region of the SSU rRNA gene
freshwater ciliates
morphospecies counts
planktonic ciliates
seasonality
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2019
2019
Historique:
received:
09
11
2018
accepted:
30
01
2019
entrez:
7
3
2019
pubmed:
7
3
2019
medline:
7
3
2019
Statut:
epublish
Résumé
Ciliates represent central nodes in freshwater planktonic food webs, and many species show pronounced seasonality, with short-lived maxima of a few dominant taxa while many being rare or ephemeral. These observations are primarily based on morphospecies counting methods, which, however, have limitations concerning the amount and volume of samples that can be processed. For high sampling frequencies at large scales, high throughput sequencing (HTS) of freshwater ciliates seems to be a promising tool. However, several studies reported large discrepancy between species abundance determinations by molecular compared to morphological means. Therefore, we compared ciliate DNA metabarcodes (V9 regions of the 18S rRNA gene) with morphospecies counts for a 3-year study (Lake Zurich, Switzerland; biweekly sampling,
Identifiants
pubmed: 30837972
doi: 10.3389/fmicb.2019.00248
pmc: PMC6389714
doi:
Types de publication
Journal Article
Langues
eng
Pagination
248Subventions
Organisme : Austrian Science Fund FWF
ID : I 2238
Pays : Austria
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