Dynamic protein changes in the perihaemorrhagic zone of Surgically Treated Intracerebral Haemorrhage Patients.
Adult
Aged
Biomarkers
/ metabolism
Brain Injuries
/ genetics
Cerebellar Cortex
/ metabolism
Cerebral Hemorrhage
/ genetics
Chromatography, Liquid
Electrophoresis, Gel, Two-Dimensional
Female
Glucose
/ metabolism
Humans
Male
Microdialysis
Middle Aged
Proteins
/ genetics
Proteomics
Tandem Mass Spectrometry
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 02 2019
28 02 2019
Historique:
received:
28
08
2018
accepted:
10
01
2019
entrez:
1
3
2019
pubmed:
1
3
2019
medline:
17
9
2020
Statut:
epublish
Résumé
The secondary injury cascades exacerbating the initial brain injury following intracerebral haemorrhage (ICH) are incompletely understood. We used dual microdialysis (MD) catheters placed in the perihaemorrhagic zone (PHZ) and in seemingly normal cortex (SNX) at time of surgical ICH evacuation in ten patients (range 26-70 years). Routine interstitial MD markers (including glucose and the lactate/pyruvate ratio) were analysed and remaining microdialysate was analysed by two-dimensional gel electrophoresis (2-DE) and nano-liquid chromatography tandem mass spectrometry (nLC-MS/MS). Two time intervals were analysed; median 2-10 hours post-surgery (time A) and median 68-76 hours post-ICH onset (time B). Using 2-DE, we quantified 232 ± 31 different protein spots. Two proteins differed between the MD catheters at time A, and 12 proteins at time B (p < 0.05). Thirteen proteins were significantly altered between time A and time B in the SNX and seven proteins in the PHZ, respectively. Using nLC-MS/MS ca 800 proteins were identified out of which 76 were present in all samples. At time A one protein was upregulated and two downregulated, and at time B, seven proteins were upregulated, and four downregulated in the PHZ compared to the SNX. Microdialysis-based proteomics is feasible for study of secondary injury mechanisms and discovery of biomarkers after ICH.
Identifiants
pubmed: 30816204
doi: 10.1038/s41598-019-39499-2
pii: 10.1038/s41598-019-39499-2
pmc: PMC6395593
doi:
Substances chimiques
Biomarkers
0
Proteins
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3181Références
Neurosurg Clin N Am. 2002 Jul;13(3):355-70
pubmed: 12486925
Lancet. 2009 May 9;373(9675):1632-44
pubmed: 19427958
Intensive Care Med. 2015 Sep;41(9):1517-28
pubmed: 26194024
Neurology. 1999 Jan 15;52(2):266-72
pubmed: 9932942
Clin Proteomics. 2017 Apr 26;14:14
pubmed: 28450824
J Mol Neurosci. 2018 May;65(1):28-34
pubmed: 29700768
Biol Psychiatry. 2007 Oct 15;62(8):847-55
pubmed: 17544382
Front Immunol. 2018 Sep 11;9:1921
pubmed: 30254628
Behav Neurol. 2018 Jul 9;2018:7646104
pubmed: 30123388
Neurocrit Care. 2014 Dec;21 Suppl 2:S148-58
pubmed: 25208673
Lancet. 2013 Aug 3;382(9890):397-408
pubmed: 23726393
Electrophoresis. 2000 Apr;21(6):1037-53
pubmed: 10786879
Neurobiol Aging. 2017 Nov;59:10-14
pubmed: 28780366
Cell Death Differ. 2016 Nov 1;23(11):1749-1764
pubmed: 27518433
Acta Neurochir (Wien). 2006 Mar;148(3):319-24; discussion 324
pubmed: 16411015
J Proteome Res. 2006 Sep;5(9):2339-47
pubmed: 16944946
Neurosurgery. 2006 Sep;59(3):521-8; discussion 521-8
pubmed: 16955033
Amyloid. 2008 Dec;15(4):255-61
pubmed: 19065297
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
J Mol Neurosci. 2018 Dec;66(4):639-648
pubmed: 30430305
Stroke. 2015 Jul;46(7):2032-60
pubmed: 26022637
PLoS One. 2012;7(12):e52560
pubmed: 23300707
J Neurochem. 2010 Dec;115(6):1434-44
pubmed: 21044072
Neuroscience. 2013 Jan 3;228:190-9
pubmed: 23085216
Sci Rep. 2017 Mar 07;7:43512
pubmed: 28266628
J Neurosurg. 2005 Mar;102(3):460-9
pubmed: 15796380
Neurosurgery. 2005 Jun;56(6):1264-8; discussion 1268-70
pubmed: 15918942
Brain Behav. 2018 Nov;8(11):e01130
pubmed: 30307711
Brain Res Rev. 2006 Aug;51(2):212-39
pubmed: 16368148
Crit Care Med. 2005 Feb;33(2):407-13
pubmed: 15699846
Physiol Meas. 2005 Aug;26(4):423-8
pubmed: 15886437
Neurocrit Care. 2010 Jun;12(3):324-36
pubmed: 20225002
Stroke. 2006 Oct;37(10):2457-62
pubmed: 16960094
J Cereb Blood Flow Metab. 2007 May;27(5):894-908
pubmed: 17033693
Lancet. 2005 Jan 29-Feb 4;365(9457):387-97
pubmed: 15680453
Prog Neurobiol. 2014 Apr;115:25-44
pubmed: 24291544
J Pain Res. 2017 Mar 13;10:575-590
pubmed: 28331360
Prog Neurobiol. 2010 Dec;92(4):463-77
pubmed: 20713126
Int J Stroke. 2014 Oct;9(7):840-55
pubmed: 25156220
Neurochem Res. 2016 Apr;41(4):748-57
pubmed: 26498936
Front Cell Neurosci. 2014 Nov 20;8:388
pubmed: 25477782
Stroke. 1988 May;19(5):604-7
pubmed: 3363593
Anal Chem. 1996 Mar 1;68(5):850-8
pubmed: 8779443
J Neurotrauma. 2004 Feb;21(2):125-36
pubmed: 15000754
Curr Opin Crit Care. 2006 Apr;12(2):112-8
pubmed: 16543785
Neurosurgery. 2019 Jun 1;84(6):1269-1279
pubmed: 29788388
Stroke. 2009 Oct;40(10):3393-5
pubmed: 19679846
Arch Neurol. 2010 May;67(5):570-6
pubmed: 20457956
J Neurosurg. 2001 Mar;94(3):397-402
pubmed: 11235942
J Stroke Cerebrovasc Dis. 2015 Dec;24(12):2800-9
pubmed: 26403367
J Appl Physiol (1985). 2001 Dec;91(6):2785-801
pubmed: 11717247
Anal Chem. 2010 Jun 1;82(11):4376-85
pubmed: 20465223
J Neurol Sci. 2010 Aug 15;295(1-2):16-22
pubmed: 20557898
Cochrane Database Syst Rev. 2008 Oct 08;(4):CD000200
pubmed: 18843607
Sci Rep. 2018 May 1;8(1):6807
pubmed: 29717219
Mol Biosyst. 2013 Nov;9(11):2589-96
pubmed: 23999822
Eur J Neurol. 2018 Oct;25(10):1195-1196
pubmed: 29956415
J Intern Med. 1991 Oct;230(4):365-73
pubmed: 1919432
J Neurosci. 2009 Dec 16;29(50):15819-27
pubmed: 20016097
Acta Neurochir Suppl. 2011;111:107-12
pubmed: 21725739