Quality and quantity of dromedary camel DNA sampled from whole-blood, saliva, and tail-hair.

Animals Biological Specimen Banks / standards Blood / metabolism Camelus / genetics DNA / analysis Hair / chemistry Saliva / chemistry Specimen Handling / methods

Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2019
Historique:
received: 15 10 2018
accepted: 18 01 2019
entrez: 1 2 2019
pubmed: 1 2 2019
medline: 13 11 2019
Statut: epublish

Résumé

Camels are livestock with unique adaptations to hot-arid regions. To effectively study camel traits, a biobank of camel DNA specimens with associated biological information is needed. We examined whole-blood, saliva (buccal swabs), and tail-hair follicle samples to determine which is the best source for establishing a DNA biobank. We inspected five amounts of each of whole-blood, buccal swabs, and tail-hair follicles in nine camels, both qualitatively via gel electrophoresis and quantitatively using a NanoDrop spectrophotometer. We also tested the effects of long term-storage on the quality and quantity of DNA, and measured the rate of degradation, by analyzing three buccal swab samples and 30 tail-hair follicles over a period of nine months. Good quality DNA, in the form of visible large size DNA bands, was extracted from all three sources, for all five amounts. The five volumes of whole-blood samples (20-100μl) provided ~0.4-3.6 μg, the five quantities of buccal swabs (1-5) produced ~0.1-12 μg, while the five amounts of tail-hair follicles (10-50) resulted in ~0.7-25 μg. No differences in the rate of degradation of buccal swab and tail-hair follicle DNA were detected, but there was clearly greater deterioration in the quality of DNA extracted from buccal swabs when compared to tail-hair follicles. We recommend using tail-hair samples for camel DNA biobanking, because it resulted in both an adequate quality and quantity of DNA, along with its ease of collection, transportation, and storage. Compared to its success in studies of other domesticated animals, we anticipate that using ~50 tail-hair follicles will provide sufficient DNA for sequencing or SNP genotyping.

Identifiants

DOI: 10.1371/journal.pone.0211743 PMID: 30703133 PMC: PMC6355012
pubmed: 30703133
doi: 10.1371/journal.pone.0211743
pii: PONE-D-18-29862
pmc: PMC6355012
doi:

Substances chimiques

DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0211743

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Hasan Alhaddad (H)

Department of Biological Sciences, Kuwait University, Safat, Kuwait.
ORCID: 0000-0001-7904-2913

Tasneem Maraqa (T)

Department of Biological Sciences, Kuwait University, Safat, Kuwait.

Suha Alabdulghafour (S)

Department of Biological Sciences, Kuwait University, Safat, Kuwait.

Huda Alaskar (H)

Department of Biological Sciences, Kuwait University, Safat, Kuwait.
ORCID: 0000-0002-5330-4919

Randa Alaqeely (R)

Department of Biological Sciences, Kuwait University, Safat, Kuwait.

Faisal Almathen (F)

Department of Veterinary Public Health and Animal Husbandry, College of Veterinary Medicine, King Faisal University, Al-Hasa, Saudi Arabia.
The Camel Research Center, King Faisal University, Al-Hasa, Saudi Arabia.

Bader H Alhajeri (BH)

Department of Biological Sciences, Kuwait University, Safat, Kuwait.

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