Inhibitory effects of azithromycin on the adherence ability of Porphyromonas gingivalis.

antibiotic antimicrobial gingivitis microbiology oral medicine pathogenesis of periodontal disease periodontitis

Journal

Journal of periodontology
ISSN: 1943-3670
Titre abrégé: J Periodontol
Pays: United States
ID NLM: 8000345

Informations de publication

Date de publication:
08 2019
Historique:
received: 25 09 2018
revised: 07 12 2018
accepted: 24 12 2018
pubmed: 29 1 2019
medline: 9 4 2020
entrez: 29 1 2019
Statut: ppublish

Résumé

Porphyromonas gingivalis is a major pathogen and has a high detection rate in periodontal disease. Fimbriae and hemagglutinin are expressed by P. gingivalis, and these play an important role in the adherence of the bacteria to periodontal tissue and biofilm formation. The aim of this study was to investigate the effects of sub-minimal inhibitory concentrations (sub-MICs) of azithromycin on the adherence of P. gingivalis, focusing on the inhibition of fimbriae expression and hemagglutinin activity. P. gingivalis ATCC 33277 were incubated anaerobically with sub-MICs of azithromycin at 37°C by gentle shaking for 18 hours. The bacterial cells were harvested, washed twice with phosphate-buffered saline (PBS), and the proteins analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. Adherence assay and hemagglutinin activity tests were done with the same culture. The results of SDS-PAGE indicated that the sub-MICs of azithromycin inhibited 41-kDa fimbrial protein expression and hemagglutinin activities. The disappearance of 41-kDa fimbrial protein expression and long fimbriae in 0.4 µg/mL, 0.2 µg/mL, and 0.1 µg/mL of azithromycin was confirmed by western blotting and transmission electron microscopy. The adherence of P. gingivalis to human gingival epithelial cells was reduced by sub-MICs of azithromycin compared with the adherence levels without antibiotic. These results suggest that sub-MICs of azithromycin may reduce the adherence of P. gingivalis to host cells, by inhibiting production of fimbriae and hemagglutinin activities. Therefore, azithromycin can be used as a biofilm treatment of periodontal disease caused by P. gingivalis.

Sections du résumé

BACKGROUND
Porphyromonas gingivalis is a major pathogen and has a high detection rate in periodontal disease. Fimbriae and hemagglutinin are expressed by P. gingivalis, and these play an important role in the adherence of the bacteria to periodontal tissue and biofilm formation. The aim of this study was to investigate the effects of sub-minimal inhibitory concentrations (sub-MICs) of azithromycin on the adherence of P. gingivalis, focusing on the inhibition of fimbriae expression and hemagglutinin activity.
METHODS
P. gingivalis ATCC 33277 were incubated anaerobically with sub-MICs of azithromycin at 37°C by gentle shaking for 18 hours. The bacterial cells were harvested, washed twice with phosphate-buffered saline (PBS), and the proteins analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting. Adherence assay and hemagglutinin activity tests were done with the same culture.
RESULTS
The results of SDS-PAGE indicated that the sub-MICs of azithromycin inhibited 41-kDa fimbrial protein expression and hemagglutinin activities. The disappearance of 41-kDa fimbrial protein expression and long fimbriae in 0.4 µg/mL, 0.2 µg/mL, and 0.1 µg/mL of azithromycin was confirmed by western blotting and transmission electron microscopy. The adherence of P. gingivalis to human gingival epithelial cells was reduced by sub-MICs of azithromycin compared with the adherence levels without antibiotic.
CONCLUSIONS
These results suggest that sub-MICs of azithromycin may reduce the adherence of P. gingivalis to host cells, by inhibiting production of fimbriae and hemagglutinin activities. Therefore, azithromycin can be used as a biofilm treatment of periodontal disease caused by P. gingivalis.

Identifiants

pubmed: 30690740
doi: 10.1002/JPER.18-0559
doi:

Substances chimiques

Bacterial Proteins 0
Fimbriae Proteins 147680-16-8
Azithromycin 83905-01-5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

903-910

Informations de copyright

© 2019 American Academy of Periodontology.

Auteurs

Powen Kan (P)

Division of Periodontology, Department of Oral Interdisciplinary Medicine, Kanagawa Dental University, Yokosuka, Japan.

Haruka Sasaki (H)

Division of Microbiology, Department of Oral Science, Kanagawa Dental University, Yokosuka, Japan.

Keitaro Inaba (K)

Division of Microbiology, Department of Oral Science, Kanagawa Dental University, Yokosuka, Japan.

Kiyoko Watanabe (K)

Division of Microbiology, Department of Oral Science, Kanagawa Dental University, Yokosuka, Japan.

Nobushiro Hamada (N)

Division of Microbiology, Department of Oral Science, Kanagawa Dental University, Yokosuka, Japan.

Masato Minabe (M)

Division of Periodontology, Department of Oral Interdisciplinary Medicine, Kanagawa Dental University, Yokosuka, Japan.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH