The Effect of Transfusion-Dependent Thalassemia Patient's Serum on Peripheral Blood Mononuclear Cell Viability.
Transfusion-dependent thalassemia
iron
peripheral blood mononuclear cell viability
thalassemia
Journal
Journal of cell death
ISSN: 1179-0660
Titre abrégé: J Cell Death
Pays: United States
ID NLM: 101534028
Informations de publication
Date de publication:
2019
2019
Historique:
received:
28
11
2018
accepted:
10
12
2018
entrez:
24
1
2019
pubmed:
24
1
2019
medline:
24
1
2019
Statut:
epublish
Résumé
Iron overload is a major complication in transfusion-dependent thalassemia (TDT) patients. Chronic oxidative stress from iron overload may lead to cellular damage and viability. This is a cross-sectional study. Transfusion-dependent thalassemia patients aged ⩾18 years old were enrolled. Transfusion-dependent thalassemia patient's serum and normal volunteer's serum were separately incubated with healthy peripheral blood mononuclear cells (PBMCs). The cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay at 24, 48, and 72 hours. Sixty-nine TDT patients and 22 healthy controls were enrolled. The mean of PBMCs viability after incubation with serum from TDT patients was lower than that with the controls (88.65% vs 103.56% at 24 hours, 78.77% vs 112.04%% at 48 hours, and 71.18% vs 132.16%% at 72 hours, respectively). High serum ferritin level (correlation -0.29,
Identifiants
pubmed: 30670921
doi: 10.1177/1179066018823534
pii: 10.1177_1179066018823534
pmc: PMC6327338
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1179066018823534Déclaration de conflit d'intérêts
Declaration of conflicting interests:The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
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