Critical role of TLR2 in triggering protective immunity with cyclophilin entrapped in oligomannose-coated liposomes against Neospora caninum infection in mice.
Adjuvants, Immunologic
/ administration & dosage
Animals
Antibodies, Protozoan
/ blood
Antigens, Protozoan
/ administration & dosage
Cell Proliferation
Coccidiosis
/ prevention & control
Cyclophilins
/ administration & dosage
Disease Models, Animal
Drug Carriers
/ administration & dosage
Female
Immunity, Cellular
Immunity, Humoral
Immunoglobulin G
/ blood
Interferon-gamma
/ metabolism
Leukocytes, Mononuclear
/ immunology
Liposomes
/ administration & dosage
Male
Mice, Inbred BALB C
Mice, Inbred C57BL
Neospora
/ immunology
Oligosaccharides
/ administration & dosage
Protozoan Vaccines
/ administration & dosage
Survival Analysis
Toll-Like Receptor 2
/ metabolism
Cyclophilin
NcCyp
Neospora caninum
Neosporosis
Vaccination
Journal
Vaccine
ISSN: 1873-2518
Titre abrégé: Vaccine
Pays: Netherlands
ID NLM: 8406899
Informations de publication
Date de publication:
08 02 2019
08 02 2019
Historique:
received:
10
10
2018
revised:
25
12
2018
accepted:
03
01
2019
pubmed:
21
1
2019
medline:
24
6
2020
entrez:
21
1
2019
Statut:
ppublish
Résumé
Neospora caninum is an intracellular protozoan parasite closely related to Toxoplasma gondii. N. caninum is thought to be a major cause of abortion in cattle worldwide. Given the current situation of drastic economic losses and a lack of efficient control strategies against such parasites, the challenge to develop potent vaccine candidates and technologies remains. We investigated the immune stimulating activity of N. caninum cyclophilin (NcCyp) with and without a formulation with oligomannose-coated-liposomes (OML) as the potential adjuvant. NcCyp-OML activated NF-κB in RAW 264.7 cells and triggered interleukin (IL)-12p40 production from murine peritoneal macrophages. In BALB/c mice, immunization with NcCyp-OML was associated with the production of specific antibodies (IgG1 and IgG2a). The specific antibody (IgG1) against naked NcCyp was also observed after the challenge infection, but it was significantly lower than those of NcCyp-OML. Moreover, significant cellular immune responses were induced, including spleen cell proliferation and interferon-gamma production. The immunization of mice with NcCyp-OML, and to lesser extent with naked NcCyp, induced significant protection against challenge with a lethal dose of N. caninum compared with the PBS control group. This protection was associated with a higher survival rate, slight changes in body weight, and lower clinical score of mice. In addition, the significant protective efficacy of NcCyp-OML was confirmed in another mouse strain, male C57BL/6 mice. The current study revealed the marked contribution of Toll-like receptor 2 (TLR2) to the protective immunity triggered by NcCyp-OML because higher numbers of TLR2
Identifiants
pubmed: 30660401
pii: S0264-410X(19)30036-2
doi: 10.1016/j.vaccine.2019.01.005
pii:
doi:
Substances chimiques
Adjuvants, Immunologic
0
Antibodies, Protozoan
0
Antigens, Protozoan
0
Drug Carriers
0
Immunoglobulin G
0
Liposomes
0
Oligosaccharides
0
Protozoan Vaccines
0
Tlr2 protein, mouse
0
Toll-Like Receptor 2
0
Interferon-gamma
82115-62-6
Cyclophilins
EC 5.2.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
937-944Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.