The nucleoid-associated protein WHIRLY1 is required for the coordinate assembly of plastid and nucleus-encoded proteins during chloroplast development.
Barley
Chlorophyll b
LHC
Pigment–protein complexes
Plastid ribosomes
Plastid signaling
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
May 2019
May 2019
Historique:
received:
29
08
2018
accepted:
21
12
2018
pubmed:
12
1
2019
medline:
14
5
2019
entrez:
12
1
2019
Statut:
ppublish
Résumé
Chloroplasts deficient in the major chloroplast nucleoid-associated protein WHIRLY1 have an enhanced ratio of LHCs to reaction centers, indicating that WHIRLY1 is required for a coordinate assembly of the photosynthetic apparatus during chloroplast development. Chloroplast development was found to be delayed in barley plants with an RNAi-mediated knockdown of WHIRLY1 encoding a major nucleoid-associated protein of chloroplasts. The plastids of WHIRLY1 deficient plants had a reduced ribosome content. Accordingly, plastid-encoded proteins of the photosynthetic apparatus showed delayed accumulation during chloroplast development coinciding with a delayed increase in photosystem II efficiency measured by chlorophyll fluorescence. In contrast, light harvesting complex proteins being encoded in the nucleus had a high abundance as in the wild type. The unbalanced assembly of the proteins of the photosynthetic apparatus in WHIRLY1-deficient plants coincided with the enhanced contents of chlorophyll b and xanthophylls. The lack of coordination was most obvious at the early stages of development. Overaccumulation of LHC proteins in comparison to reaction center proteins at the early stages of chloroplast development did not correlate with enhanced expression levels of the corresponding genes in the nucleus. This work revealed that WHIRLY1 does not influence LHC abundance at the transcriptional level. Rather, WHIRLY1 in association with nucleoids might play a structural role for both the assembly of ribosomes and the complexes of the photosynthetic apparatus.
Identifiants
pubmed: 30631956
doi: 10.1007/s00425-018-03085-z
pii: 10.1007/s00425-018-03085-z
doi:
Substances chimiques
Plant Proteins
0
Chlorophyll
1406-65-1
chlorophyll b
5712ZB110R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1337-1347Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : KR 1350/19-1
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