Human Monocyte Heterogeneity as Revealed by High-Dimensional Mass Cytometry.
atherosclerosis
biomarkers
coronary artery disease
mass cytometry
monocytes
Journal
Arteriosclerosis, thrombosis, and vascular biology
ISSN: 1524-4636
Titre abrégé: Arterioscler Thromb Vasc Biol
Pays: United States
ID NLM: 9505803
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
pubmed:
26
12
2018
medline:
4
12
2019
entrez:
25
12
2018
Statut:
ppublish
Résumé
Objective- Three distinct human monocyte subsets have been identified based on the surface marker expression of CD14 and CD16. We hypothesized that monocytes were likely more heterogeneous in composition. Approach and Results- We used the high dimensionality of mass cytometry together with the FlowSOM clustering algorithm to accurately identify and define monocyte subsets in blood of healthy human subjects and those with coronary artery disease (CAD). To study the behavior and functionality of the newly defined monocyte subsets, we performed RNA sequencing, transwell migration, and efferocytosis assays. Here, we identify 8 human monocyte subsets based on their surface marker phenotype. We found that 3 of these subsets fall within the CD16
Identifiants
pubmed: 30580568
doi: 10.1161/ATVBAHA.118.311022
pmc: PMC6697379
mid: NIHMS1035194
doi:
Substances chimiques
Lipopolysaccharide Receptors
0
Receptors, IgG
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
25-36Subventions
Organisme : NCI NIH HHS
ID : U01 CA224766
Pays : United States
Organisme : NIH HHS
ID : S10 OD016262
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR027366
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL136275
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL055798
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL134236
Pays : United States
Organisme : NIH HHS
ID : S10 OD018499
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA202987
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL107490
Pays : United States
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