Versatile Electroanalytical Bioplatforms for Simultaneous Determination of Cancer-Related DNA 5-Methyl- and 5-Hydroxymethyl-Cytosines at Global and Gene-Specific Levels in Human Serum and Tissues.
5-Methylcytosine
/ analogs & derivatives
Antibodies, Monoclonal
/ immunology
Armoracia
/ enzymology
Biomarkers, Tumor
/ blood
Biosensing Techniques
/ methods
DNA
/ blood
DNA Methylation
DNA Modification Methylases
/ genetics
DNA Repair Enzymes
/ genetics
Electrochemical Techniques
/ methods
Fluorescent Dyes
/ chemistry
Horseradish Peroxidase
/ chemistry
Humans
Hydrogen Peroxide
/ chemistry
Immunomagnetic Separation
Limit of Detection
Tumor Suppressor Proteins
/ genetics
5-hmC
5-mC
cancer
electrochemical platforms
global and gene-specific DNA methylation
human serum
paraffin-embedded colorectal tissues
Journal
ACS sensors
ISSN: 2379-3694
Titre abrégé: ACS Sens
Pays: United States
ID NLM: 101669031
Informations de publication
Date de publication:
25 01 2019
25 01 2019
Historique:
pubmed:
1
12
2018
medline:
29
1
2020
entrez:
1
12
2018
Statut:
ppublish
Résumé
This paper reports the preparation of versatile electrochemical biosensing platforms for the simple, rapid, and PCR-independent detection of the most frequent DNA methylation marks (5-methylcytosine, 5-mC, and/or 5-hydroxymethylcytosine, 5-hmC) both at global and gene-specific levels. The implemented strategies, relying on the smart coupling of immuno-magnetic beads (MBs), specific DNA probes and amperometric detection at screen-printed carbon electrodes (SPCEs), provided sensitive and selective determination of the target methylated DNAs in less than 90 min with a great reproducibility and demonstrated feasibility for the simultaneous detection of the same or different cytosine epimarks both at global level and in different loci of the same gene or in different genes. The bioplatforms were applied to determine global methylation events in paraffin-embedded colorectal tissues and specific methylation at promoters of tumor suppressor genes in genomic DNA extracted from cancer cells and paraffin-embedded colorectal tissues, and in serum without previous DNA extraction from cancer patients.
Identifiants
pubmed: 30499292
doi: 10.1021/acssensors.8b01339
doi:
Substances chimiques
Antibodies, Monoclonal
0
Biomarkers, Tumor
0
Fluorescent Dyes
0
RASSF1 protein, human
0
Tumor Suppressor Proteins
0
5-hydroxymethylcytosine
1123-95-1
5-Methylcytosine
6R795CQT4H
DNA
9007-49-2
Hydrogen Peroxide
BBX060AN9V
Horseradish Peroxidase
EC 1.11.1.-
DNA Modification Methylases
EC 2.1.1.-
MGMT protein, human
EC 2.1.1.63
DNA Repair Enzymes
EC 6.5.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM