Transcriptional Profiling of Dendritic Cells in a Mouse Model of Food-Antigen-Induced Anaphylaxis Reveals the Upregulation of Multiple Immune-Related Pathways.


Journal

Molecular nutrition & food research
ISSN: 1613-4133
Titre abrégé: Mol Nutr Food Res
Pays: Germany
ID NLM: 101231818

Informations de publication

Date de publication:
02 2019
Historique:
received: 26 07 2018
revised: 05 11 2018
pubmed: 21 11 2018
medline: 14 6 2019
entrez: 21 11 2018
Statut: ppublish

Résumé

Much of the knowledge about gene expression during anaphylaxis comes from candidate gene studies. Despite their potential role, expression changes in dendritic cells (DCs) have not been studied in this context using high throughput methods. The molecular mechanisms underlying food-antigen-induced anaphylaxis are investigated using DCs from an animal model. RNA sequencing is used to study gene expression in lymph-node-derived DCs from anaphylactic mice sensitized intranasally with the major peach allergen Pru p 3 during the acute reaction phase, induced intraperitoneally. In total, 237 genes changed significantly, 181 showing at least twofold changes. Almost three-quarters of these increase during anaphylaxis. A subset is confirmed using RT-PCR in a second set of samples obtained from a new batch of mice. Enrichment analysis shows an overrepresentation of genes involved in key immune system and inflammatory processes, including TGF-β signaling. Comparison with a study using anaphylactic human subjects show significant overlap. The findings provide a comprehensive overview of the transcriptional changes occurring in DCs during anaphylaxis and help elucidate the mechanisms involved. They add further weight to the putative role of these cells in anaphylaxis and highlight genes that may represent potential therapeutic targets.

Identifiants

pubmed: 30458065
doi: 10.1002/mnfr.201800759
doi:

Substances chimiques

Antigens, Plant 0
Plant Proteins 0
Pru p 3 allergen 0
Interleukin-10 130068-27-8
Immunoglobulin E 37341-29-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1800759

Informations de copyright

© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Auteurs

Maria Jose Rodriguez (MJ)

Research Laboratory, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.

Francisca Palomares (F)

Research Laboratory, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.

Gador Bogas (G)

Allergy Unit, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.

Maria Jose Torres (MJ)

Allergy Unit, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.

Araceli Diaz-Perales (A)

Centro de Biotecnología y Genómica de Plantas (CBGP, UPM-INIA), Universidad Politécnica de Madrid (UPM), 28223, Pozuelo de Alarcon, Spain.

Javier Rojo (J)

Laboratory, Instituto de Investigaciones Químicas (IIQ), CSIC-Universidad de Sevilla, 41092, Sevilla, Spain.

Maria Del Carmen Plaza-Seron (MDC)

Research Laboratory, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.

Alba Rodriguez-Nogales (A)

Research Laboratory, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.

Christine Orengo (C)

Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, WC1E 6BT, UK.

Cristobalina Mayorga (C)

Research Laboratory, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.
Allergy Unit, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.

James Richard Perkins (JR)

Research Laboratory, IBIMA-Regional University Hospital of Malaga, UMA, 29009, Malaga, Spain.

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Classifications MeSH