Transcriptional Profiling of Dendritic Cells in a Mouse Model of Food-Antigen-Induced Anaphylaxis Reveals the Upregulation of Multiple Immune-Related Pathways.
Anaphylaxis
/ etiology
Animals
Antigens, Plant
/ immunology
Dendritic Cells
/ immunology
Female
Food Hypersensitivity
/ etiology
Gene Expression Profiling
Gene Ontology
Humans
Immunoglobulin E
/ blood
Interleukin-10
/ physiology
Mice
Mice, Inbred BALB C
Plant Proteins
/ immunology
Sequence Analysis, RNA
Up-Regulation
anaphylaxis
dendritic cells
food allergy
lipid transfer proteins
transcriptomics
Journal
Molecular nutrition & food research
ISSN: 1613-4133
Titre abrégé: Mol Nutr Food Res
Pays: Germany
ID NLM: 101231818
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
26
07
2018
revised:
05
11
2018
pubmed:
21
11
2018
medline:
14
6
2019
entrez:
21
11
2018
Statut:
ppublish
Résumé
Much of the knowledge about gene expression during anaphylaxis comes from candidate gene studies. Despite their potential role, expression changes in dendritic cells (DCs) have not been studied in this context using high throughput methods. The molecular mechanisms underlying food-antigen-induced anaphylaxis are investigated using DCs from an animal model. RNA sequencing is used to study gene expression in lymph-node-derived DCs from anaphylactic mice sensitized intranasally with the major peach allergen Pru p 3 during the acute reaction phase, induced intraperitoneally. In total, 237 genes changed significantly, 181 showing at least twofold changes. Almost three-quarters of these increase during anaphylaxis. A subset is confirmed using RT-PCR in a second set of samples obtained from a new batch of mice. Enrichment analysis shows an overrepresentation of genes involved in key immune system and inflammatory processes, including TGF-β signaling. Comparison with a study using anaphylactic human subjects show significant overlap. The findings provide a comprehensive overview of the transcriptional changes occurring in DCs during anaphylaxis and help elucidate the mechanisms involved. They add further weight to the putative role of these cells in anaphylaxis and highlight genes that may represent potential therapeutic targets.
Identifiants
pubmed: 30458065
doi: 10.1002/mnfr.201800759
doi:
Substances chimiques
Antigens, Plant
0
Plant Proteins
0
Pru p 3 allergen
0
Interleukin-10
130068-27-8
Immunoglobulin E
37341-29-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1800759Informations de copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.