Fib3-3 as a Biomarker for Osteoarthritis in a Rat Model with Metabolic Dysregulation.
animal model
articular cartilage
osteoarthritis; biomarker
Journal
Cartilage
ISSN: 1947-6043
Titre abrégé: Cartilage
Pays: United States
ID NLM: 101518378
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
pubmed:
26
1
2018
medline:
25
3
2020
entrez:
26
1
2018
Statut:
ppublish
Résumé
Fibulin-3 is a glycoprotein highly expressed in osteoarthritic cartilage and inhibits angiogenesis and chondrocyte differentiation. Recent studies have indicated that fibulin-3 has potential value as a biomarker in osteoarthritis. The aim of the present study is to examine the role of 3 fibulin-3 peptides (Fib3-1, Fib3-2, and Fib3-3) and a type II collagen degradation product in a rat osteoarthritis model with systemic metabolic alterations combined with local cartilage damage. Forty, 12-week-old male, Wistar rats were randomly divided over 2 groups: a standard or a high-fat diet inducing metabolic dysregulation. After 12 weeks, articular cartilage damage was induced on the femoral condyles (groove model), in 1 knee joint in 14 rats of each diet group. At endpoint, blood was collected and serum was isolated. Enzyme-linked immunosorbent assay on all selected fibulin-3 fragments was performed from serum samples in addition to immunohistochemical analysis for Fib3-3. Serum concentrations of Fib3-3 were increased by 29.9%, when cartilage damage was induced in addition to a high-fat diet. Fib3-3 was also associated with an increased histological total joint degeneration ( In the rat groove model combined with high-fat diet-induced metabolic dysregulation an increased Fib3-3 concentration was observed systemically, which is associated with local joint degeneration. This suggests that systemic Fib3-3 concentrations can indicate the status of joint degeneration and function as a biomarker in osteoarthritis.
Identifiants
pubmed: 29366335
doi: 10.1177/1947603518754629
pmc: PMC6585292
doi:
Substances chimiques
Biomarkers
0
Collagen Type II
0
EFEMP1 protein, human
0
Extracellular Matrix Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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